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	Provedor de dados PAB (2) Autor Mendes,Beatriz Madalena Januzzi (2) Stipp,Liliane Cristina Libório (2) Attílio,Lísia Borges (1) Harakava,Ricardo (1) Krug,Maria Graziela Zagatto (1) Miyata,Luzia Yuriko (1) Mourão Filho,Francisco de Assis Alves (1) Rodriguez,Adriana Pinheiro Martinelli (1) Silva,Tatiane Loureiro da (1) Mais... Palavra-chave Adventitious bud (1) Antimicrobial peptide (1) Citrullus lanatus (1) Citrus sinensis (1) Histological study (1) Tissue‑specific promoter (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2005 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter PAB Attílio,Lísia Borges; Mourão Filho,Francisco de Assis Alves; Harakava,Ricardo; Silva,Tatiane Loureiro da; Miyata,Luzia Yuriko; Stipp,Liliane Cristina Libório; Mendes,Beatriz Madalena Januzzi. The objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co‑cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real‑time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events. Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Antimicrobial peptide; Tissue‑specific promoter. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000700006 In vitro organogenesis in watermelon cotyledons PAB Krug,Maria Graziela Zagatto; Stipp,Liliane Cristina Libório; Rodriguez,Adriana Pinheiro Martinelli; Mendes,Beatriz Madalena Januzzi. The objective of this work was to study the in vitro organogenesis of Citrullus lanatus, by the induction of adventitious buds in cotyledon segments cultured in medium supplemented with cytokinin. Explants were collected from one, three and five-day-old in vitro germinated seedlings, considering the distal and proximal cotyledon regions. The data obtained showed that in vitro organogenesis of watermelon occurred with higher efficiency, when cotyledon segments from the proximal region collected from three-day-old seedlings were cultivated in medium MS, supplemented with BAP (1 mg L-1) and coconut water (10%). The histological study showed that the organogenesis occurs directly, without callus formation, on epidermal and subepidermal layers of the explants.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrullus lanatus; Histological study; Adventitious bud. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000900004 Registros recuperados: 2 Primeira ... 1 ... Última

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